PSIII-19 - Effect of dietary clay mineral and organic selenium supplementation on broilers

Abstract: Selenium is an essential trace element that plays several important biological roles in humans, including regulation of the antioxidant defense system, improving immunity, and maintaining body homeostasis. However, in countries with densely populated populations around the world, selenium intake is lower than the recommended daily intake due to a lack of selenium in the soil. So, we hypothesized that the addition of organic selenium and clay minerals to broiler feed would increase selenium concentration in the meat as well as antioxidant capacity and immune response. Therefore, this study analyzed the effects of clay minerals and organotaselenium on growh performance, digestibility, intestinal morphology, blood characteristics, fecal microbiome, foot-pad dermatitis and meat quality characteristics of broilers. A total of 168 1day old broiler chicks(Arbor Acres) were randomly assigned to 3 groups based on the initial body weight(37.33 ± 0.24g) with 7 replicate per 8 birds (mixed sex). The experiments period was 28d. Dietary treatments were folloewd: Basal diets based on corn-soybean meal (Control group), basal diet supplemented with 1000ppm zeolite and 0.2ppm seleno-methionine (Treatment 1), and basal diet supplemented with 1000ppm zeolite and 0.4ppm seleno-methionine (Tretment 2). Supplementation of zeolite and seleno-methionine did not affect (p > 0.05)on growth performance, nutrient digestibility, foot-pad dermatitis. However, increasing level of seleno-metionine showed linear increase (p < 0.05) in villus height/crypt depth ratio of duodenum and selenium concentration in breast meat. Also, increasing levle of seleno-methionine showed linear decrease (p < 0.05) in blood uric acid. Total genomic DNA was used to assess the fecal microbiota diversity and composition using the V3-V4 region of the 16S rRNA gene. There were significant differences in alpha diversity and Beta diversity in fecal microbiomes among treatments (p < 0.05). Taxonomic analysis at phylum level revealed that Proteobacteria and Firmicutes of Treatment 1 and Treatment 2 were significantly lower than control group. In genus level, the relative abundance of fecal lactobacillus and Enterococcus of Treatment groups were significantly higher than control group. In short, 1000ppm zeolite and 0.4ppm seleno-methionine supplementation to broiler diet supporitng positive gut microbime change, also enhancing antioxidant capacity, and concentration selenium in muscle.